coronary artery endothelial cells caec  (Cell Applications Inc)

Journal: Biomedicines

Article Title: Tumor Necrosis Factor Receptor Superfamily Member 21 Induces Endothelial-Mesenchymal Transition in Coronary Artery Endothelium of Type 2 Diabetes Mellitus

doi: 10.3390/biomedicines10061282

Figure Lengend Snippet: Identification of differentially expressed genes correlated with CAEC injury of the type 2 DM patient and the normal individual. ( A ) The morphological changes in normal and diabetic CAECs. ( B ) Flowchart of identification of potential genes correlated with HCAECs injury in type 2 DM. ( C ) Display of differential expression patterns of normal and diabetic CAECs from deep RNA sequencing by volcano plot. The 112 differentially expressed genes between diabetic and normal CAECs were analyzed using ( D ) Tox list of IPA analysis and ( E ) biologic process in DAVID database. The top seven categories of these dysregulated genes in diabetic CAECs are displayed in a pie chart. The pie chart indicates the-Log10 (false discovery rate, FDR) of each term, and the numbers that are shown at the outside of each pie segment indicates the number of genes involved in each term.

Article Snippet: Primary coronary artery endothelial cells (CAECs) of a DM patient (Cat CC-2922) and a normal individual (Cat CC-2585) (Lonza, Walkersville, MD, USA) were cultured in Medium-2 BulletkitTM (Cat CC-3162).

Techniques: Quantitative Proteomics, RNA Sequencing

Journal: Biomedicines

Article Title: Tumor Necrosis Factor Receptor Superfamily Member 21 Induces Endothelial-Mesenchymal Transition in Coronary Artery Endothelium of Type 2 Diabetes Mellitus

doi: 10.3390/biomedicines10061282

Figure Lengend Snippet: The mRNA expression of TNFRSF21 and TNFSF4 in normal and diabetic CAECs.

Article Snippet: Primary coronary artery endothelial cells (CAECs) of a DM patient (Cat CC-2922) and a normal individual (Cat CC-2585) (Lonza, Walkersville, MD, USA) were cultured in Medium-2 BulletkitTM (Cat CC-3162).

Techniques: Expressing

Journal: Biomedicines

Article Title: Tumor Necrosis Factor Receptor Superfamily Member 21 Induces Endothelial-Mesenchymal Transition in Coronary Artery Endothelium of Type 2 Diabetes Mellitus

doi: 10.3390/biomedicines10061282

Figure Lengend Snippet: Increased TNFRSF21 and TNFSF4 expression in HCAECs in type 2 DM. ( A , B ) TNFRSF21 and TNFSF4 mRNA expression in CAECs from a normal individual and type 2 DM patient ( n = 3). ( C , D ) TNFRSF21 and TNFSF4 mRNA expression in HCAECs treated with NG (5.5 mM) or HG (25 mM) for 24 h ( n = 3). ( E , F ) TNFRSF21 and TNFSF4 mRNA expression in HCAECs treated with BSA (300 μg/mL) or AGE-BSA (300 μg/mL) for 24 h ( n = 3). TNFRSF21 and TNFSF4 mRNA levels were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). ( G , H ) TNFRSF21 protein expression in the supernatant of HCAECs treated with NG, HG, BSA, and AGE-BSA for 48 h using enzyme-linked immunosorbent assay. The bar graph represents the mean ± S.E.M. * p < 0.05, *** p < 0.001 by Student’s t -test. ns: non-significance.

Article Snippet: Primary coronary artery endothelial cells (CAECs) of a DM patient (Cat CC-2922) and a normal individual (Cat CC-2585) (Lonza, Walkersville, MD, USA) were cultured in Medium-2 BulletkitTM (Cat CC-3162).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Biomedicines

Article Title: Tumor Necrosis Factor Receptor Superfamily Member 21 Induces Endothelial-Mesenchymal Transition in Coronary Artery Endothelium of Type 2 Diabetes Mellitus

doi: 10.3390/biomedicines10061282

Figure Lengend Snippet: TNFRSF21 (DR6/Fc) promoted EndoMT in CAECs of type 2 DM. ( A ) EndoMT markers, including E-cadherin, VE-cadherin, eNOS, N-cadherin, vimentin, and α-SMA levels, were assessed in HCAECs treated with BSA (300 μg/mL) or AGE-BSA (300 μg/mL) for 48 h ( n = 3). ( B ) After treatment with TNFRSF21 (10 ng/mL) for 48 h, EndoMT markers were examined in HCAECs using Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t -test.

Article Snippet: Primary coronary artery endothelial cells (CAECs) of a DM patient (Cat CC-2922) and a normal individual (Cat CC-2585) (Lonza, Walkersville, MD, USA) were cultured in Medium-2 BulletkitTM (Cat CC-3162).

Techniques: Western Blot

Journal: Biomedicines

Article Title: Tumor Necrosis Factor Receptor Superfamily Member 21 Induces Endothelial-Mesenchymal Transition in Coronary Artery Endothelium of Type 2 Diabetes Mellitus

doi: 10.3390/biomedicines10061282

Figure Lengend Snippet: TNFRSF21 induced increased permeability within HCAECs. CDH11, PCDH7 and PCDH11 mRNA were assessed in primary CAECs of normal individual and type 2 DM patient ( A – C ), in HCAECs treated with NG (5.5 mM) or HG (25 mM) for 24 h ( n = 3) ( D – F ), and in HCAECs treated with BSA (300 μg/mL) or AGE-BSA (300 μg/mL) for 24 h ( n = 3) ( G – I ). ( J – L ) The permeability of HCAECs was examined after treatment with NG, HG, BSA, AGE-BSA, normal control (NC), or TNFRSF21 (10 ng/mL) for 48 h ( n = 3) using FITC-dextran. * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t -test.

Article Snippet: Primary coronary artery endothelial cells (CAECs) of a DM patient (Cat CC-2922) and a normal individual (Cat CC-2585) (Lonza, Walkersville, MD, USA) were cultured in Medium-2 BulletkitTM (Cat CC-3162).

Techniques: Permeability, Control

Journal: Biomedicines

Article Title: Tumor Necrosis Factor Receptor Superfamily Member 21 Induces Endothelial-Mesenchymal Transition in Coronary Artery Endothelium of Type 2 Diabetes Mellitus

doi: 10.3390/biomedicines10061282

Figure Lengend Snippet: Illustration of the mechanism by which AGEs induced EndoMT and the increase in permeability in CAECs through TNFRSF21 and TNFRSF21 as a biomarker of abnormality of left ventricular structure and systolic function in type 2 DM.

Article Snippet: Primary coronary artery endothelial cells (CAECs) of a DM patient (Cat CC-2922) and a normal individual (Cat CC-2585) (Lonza, Walkersville, MD, USA) were cultured in Medium-2 BulletkitTM (Cat CC-3162).

Techniques: Permeability, Biomarker Discovery